Bradyrhizobiumcajani sp. nov. Isolated from nodules of Cajanuscajan
Bradyrhizobiumcajani sp. nov. Isolated from nodules of Cajanuscajan
No Thumbnail Available
Date
2017-07-01
Authors
César Díaz Alcántara
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Two slow-growing strains, AMBPC1010T and AMBPC1011, were isolated from nodules of Cajanus cajan in the Dominican Republic. 16S rRNA gene analysis placed these strains within the genus Bradyrhizobium , being phylogenetically equidistant to several species of this genus. Analysis of the recA and atpD genes showed that the strains isolated belong to a cluster containing the strains Bradyrhizobium ottawaense OO99T, ‘ Bradyrhizobium americanum' CMVU44 and Bradyrhizobium daqingense CCBAU 15774T, and presented similarity values lower than 96 % for both genes with respect to the strains nodulating C. cajan. DNA–DNA hybridization analysis showed averages of 36, 40 and 39 % relatedness with respect to the representative strains of Bradyrhizobium ottawaense , ‘ Bradyrhizobium americanum' and Bradyrhizobium daqingense , respectively. Phenotypic characteristics also differed from those of the most closely related species of the genus Bradyrhizobium . Therefore, based on the data obtained in this study, we propose to classify the strains AMBPC1010T (=LMG 29967T=CECT 9227T) and AMBPC1011 into a novel species named Bradyrhizobium cajani sp. nov.
Description
Keywords
Citation
Díaz-Alcántara, C. A., Santamaría, C., Temprano, F., & Ruiz-Argüeso, T. (2017). Bradyrhizobium cajani sp. nov. isolated from nodules of Cajanus cajan. International Journal of Systematic and Evolutionary Microbiology, 67(7), 2236–2241.