Development of Loop-Mediated Isothermal Amplification Assay for paid detection of Dengue Virus in aedesaegypti (Diptera:Cuclicidae) Larvae from Cuba.

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dc.contributor.authorModesto Cruz
dc.date.accessioned2026-01-27T20:18:31Z
dc.date.available2026-01-27T20:18:31Z
dc.date.issued2019-07-22
dc.description.abstractDengue fever is an arthropod-borne disease of great importance worldwide due to the number of cases, epidemics and geographical extent of its vectors. Aedes aegypti (Linnaeus) mosquito is its main vector and its chemical control is the only viable alternative to stop dengue outbreaks since there is not an effective dengue vaccine. Even though surveillance systems allow a rapid detection of dengue cases; sometimes it is not enough to prevent outbreaks due to asymptomatic cases that cannot be detected. Nine pools of field-caught Ae. aegypti larvae were collected in Havana, Cuba. They were analyzed by reverse transcription–polymerase chain reaction (RTPCR) and loop-mediated isothermal amplification (RT-LAMP) assays to detect dengue virus. From them4 out of nine were positive by RT-PCR and 9 were positive by RTLAMP with and without RNA extraction step, respectively. RT-LAMP assay, with and without RNA extraction step, was able to detect more positive samples than RT-PCR standing out as a powerful tool to dengue surveillance in mosquito populations for early prediction of outbreaks
dc.identifier.citationCruz, M., & Valdés, O. (2019). Development of loop-mediated isothermal amplification assay for rapid detection of Dengue virus in Aedes aegypti (Diptera: Culicidae) larvae from Cuba. Virology and Retrovirology Journal, 2(2), 122.
dc.identifier.urihttps://repositoriovip.uasd.edu.do/handle/123456789/1555
dc.language.isoen
dc.titleDevelopment of Loop-Mediated Isothermal Amplification Assay for paid detection of Dengue Virus in aedesaegypti (Diptera:Cuclicidae) Larvae from Cuba.
dc.typeArticle
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